negative control fopflash Search Results


topflash and fopflash system  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc topflash and fopflash system
miR-577 targets LRP6 and β-catenin. (A) A dual-luciferase reporter assay was utilized to validate the binding sites between miR-577 and LRP6 3′UTR. (B) A dual-luciferase reporter assay was utilized to validate the binding sites between miR-577 and β-catenin 3′UTR. (C) Reverse transcription-quantitative PCR was employed to detect LRP6 and β-catenin mRNA expression levels in SW1990 cells transfected with miR-577 mimics. (D) A <t>TOPFlash</t> luciferase reporter assay was performed to detect the effects of miR-577 on the activity of <t>the</t> <t>Wnt/β-catenin</t> signaling. (E and F) Pearson's correlation analysis was utilized for the correlations between (E) FGD5-AS1 and LRP6 and between (F) FGD5-AS1 and β-catenin in pancreatic cancer tissues. (G and H) Pearson's correlation analysis was utilized for the correlations between (G) miR-577 and LRP6 and between (H) miR-577 and β-catenin in pancreatic cancer tissues. All of the experiments were performed in triplicate. **P<0.01 and ***P<0.001. LRP6, low-density lipoprotein receptor-related protein 6; miR, microRNA; FGD5-AS1, FGD5 antisense RNA 1; UTR, untranslated region.
Topflash And Fopflash System, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/topflash and fopflash system/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
topflash and fopflash system - by Bioz Stars, 2026-05
90/100 stars
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negative control fopflash  (Promega)
90
Promega negative control fopflash
miR-577 targets LRP6 and β-catenin. (A) A dual-luciferase reporter assay was utilized to validate the binding sites between miR-577 and LRP6 3′UTR. (B) A dual-luciferase reporter assay was utilized to validate the binding sites between miR-577 and β-catenin 3′UTR. (C) Reverse transcription-quantitative PCR was employed to detect LRP6 and β-catenin mRNA expression levels in SW1990 cells transfected with miR-577 mimics. (D) A <t>TOPFlash</t> luciferase reporter assay was performed to detect the effects of miR-577 on the activity of <t>the</t> <t>Wnt/β-catenin</t> signaling. (E and F) Pearson's correlation analysis was utilized for the correlations between (E) FGD5-AS1 and LRP6 and between (F) FGD5-AS1 and β-catenin in pancreatic cancer tissues. (G and H) Pearson's correlation analysis was utilized for the correlations between (G) miR-577 and LRP6 and between (H) miR-577 and β-catenin in pancreatic cancer tissues. All of the experiments were performed in triplicate. **P<0.01 and ***P<0.001. LRP6, low-density lipoprotein receptor-related protein 6; miR, microRNA; FGD5-AS1, FGD5 antisense RNA 1; UTR, untranslated region.
Negative Control Fopflash, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/negative control fopflash/product/Promega
Average 90 stars, based on 1 article reviews
negative control fopflash - by Bioz Stars, 2026-05
90/100 stars
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negative control mutated tcf/lef binding site (fopflash  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc negative control mutated tcf/lef binding site (fopflash
Effects of Siah1 on <t>Tcf/Lef</t> regulated transcription activity in SKBR3 and MCF-7 cells . A: Transfected cells were cotransfected with Topflash or <t>Fopflash</t> plasmid, the Renilla luciferase reporter plasmid (pRL-TK) as an internal control and the indicated expression plasmids. Luciferase activity was measured at 24 h after transfection and plotted after normalizing with respect to the Renilla luciferase activity. Each experiment was performed at least three times. Columns, mean; bars, SD; *, p < 0.05. B; Functional inhibition of Siah-1 ubiquitin-ligase by siRNA increased TCF/Lef transcriptional activity in MCF-7 cells at 24 hours after transfection. Each experiment was performed three times. Columns, mean; bars, SD; *, Siah1 siRNA vs. both untreated and control siRNA p < 0.05.
Negative Control Mutated Tcf/Lef Binding Site (Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/negative control mutated tcf/lef binding site (fopflash/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
negative control mutated tcf/lef binding site (fopflash - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


miR-577 targets LRP6 and β-catenin. (A) A dual-luciferase reporter assay was utilized to validate the binding sites between miR-577 and LRP6 3′UTR. (B) A dual-luciferase reporter assay was utilized to validate the binding sites between miR-577 and β-catenin 3′UTR. (C) Reverse transcription-quantitative PCR was employed to detect LRP6 and β-catenin mRNA expression levels in SW1990 cells transfected with miR-577 mimics. (D) A TOPFlash luciferase reporter assay was performed to detect the effects of miR-577 on the activity of the Wnt/β-catenin signaling. (E and F) Pearson's correlation analysis was utilized for the correlations between (E) FGD5-AS1 and LRP6 and between (F) FGD5-AS1 and β-catenin in pancreatic cancer tissues. (G and H) Pearson's correlation analysis was utilized for the correlations between (G) miR-577 and LRP6 and between (H) miR-577 and β-catenin in pancreatic cancer tissues. All of the experiments were performed in triplicate. **P<0.01 and ***P<0.001. LRP6, low-density lipoprotein receptor-related protein 6; miR, microRNA; FGD5-AS1, FGD5 antisense RNA 1; UTR, untranslated region.

Journal: Oncology Reports

Article Title: FGD5-AS1 is an oncogenic lncRNA in pancreatic cancer and regulates the Wnt/β-catenin signaling pathway via miR-577

doi: 10.3892/or.2021.8232

Figure Lengend Snippet: miR-577 targets LRP6 and β-catenin. (A) A dual-luciferase reporter assay was utilized to validate the binding sites between miR-577 and LRP6 3′UTR. (B) A dual-luciferase reporter assay was utilized to validate the binding sites between miR-577 and β-catenin 3′UTR. (C) Reverse transcription-quantitative PCR was employed to detect LRP6 and β-catenin mRNA expression levels in SW1990 cells transfected with miR-577 mimics. (D) A TOPFlash luciferase reporter assay was performed to detect the effects of miR-577 on the activity of the Wnt/β-catenin signaling. (E and F) Pearson's correlation analysis was utilized for the correlations between (E) FGD5-AS1 and LRP6 and between (F) FGD5-AS1 and β-catenin in pancreatic cancer tissues. (G and H) Pearson's correlation analysis was utilized for the correlations between (G) miR-577 and LRP6 and between (H) miR-577 and β-catenin in pancreatic cancer tissues. All of the experiments were performed in triplicate. **P<0.01 and ***P<0.001. LRP6, low-density lipoprotein receptor-related protein 6; miR, microRNA; FGD5-AS1, FGD5 antisense RNA 1; UTR, untranslated region.

Article Snippet: To determine the activity of Wnt/β-catenin pathway, the TOPflash and FOPflash system (Upstate Biotechnology, Inc.) was used.

Techniques: Luciferase, Reporter Assay, Binding Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Transfection, Activity Assay

Effects of Siah1 on Tcf/Lef regulated transcription activity in SKBR3 and MCF-7 cells . A: Transfected cells were cotransfected with Topflash or Fopflash plasmid, the Renilla luciferase reporter plasmid (pRL-TK) as an internal control and the indicated expression plasmids. Luciferase activity was measured at 24 h after transfection and plotted after normalizing with respect to the Renilla luciferase activity. Each experiment was performed at least three times. Columns, mean; bars, SD; *, p < 0.05. B; Functional inhibition of Siah-1 ubiquitin-ligase by siRNA increased TCF/Lef transcriptional activity in MCF-7 cells at 24 hours after transfection. Each experiment was performed three times. Columns, mean; bars, SD; *, Siah1 siRNA vs. both untreated and control siRNA p < 0.05.

Journal: BMC Cancer

Article Title: Siah1 proteins enhance radiosensitivity of human breast cancer cells

doi: 10.1186/1471-2407-10-403

Figure Lengend Snippet: Effects of Siah1 on Tcf/Lef regulated transcription activity in SKBR3 and MCF-7 cells . A: Transfected cells were cotransfected with Topflash or Fopflash plasmid, the Renilla luciferase reporter plasmid (pRL-TK) as an internal control and the indicated expression plasmids. Luciferase activity was measured at 24 h after transfection and plotted after normalizing with respect to the Renilla luciferase activity. Each experiment was performed at least three times. Columns, mean; bars, SD; *, p < 0.05. B; Functional inhibition of Siah-1 ubiquitin-ligase by siRNA increased TCF/Lef transcriptional activity in MCF-7 cells at 24 hours after transfection. Each experiment was performed three times. Columns, mean; bars, SD; *, Siah1 siRNA vs. both untreated and control siRNA p < 0.05.

Article Snippet: The Tcf/Lef-responsive luciferase reporter gene (Topflash), the negative control with mutated Tcf/Lef binding site (Fopflash), and the Renilla luciferase reporter plasmid (pRL-TK) as an internal control were obtained from Upstate Biotechnology, USA.

Techniques: Activity Assay, Transfection, Plasmid Preparation, Luciferase, Expressing, Functional Assay, Inhibition